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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
10/11/2023 |
Actualizado : |
10/11/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BORGES, M.A.; CURCIO, B.R.; GASTAL, G.D.A.; GHENO, L.; JUNIOR, A.S.V.; CORCINI, C.D.; NOGUEIRA, C.E.W.; AGUIAR, F.L.N.; GASTAL, E.L. |
Afiliación : |
MORGANA A. BORGES, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; BRUNA R. CURCIO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIZA GHENO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; ANTONIO S. VARELA JUNIOR, Department of Comparative Animal Reproduction, Institute of Biological Sciences, Federal University of Rio Grande, RS, Rio Grande, Brazil; CARINE D. CORCINI, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; CARLOS E.W. NOGUEIRA, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; FRANCISCO L.N. AGUIAR, Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraiba, PB, Sousa, Brazil; EDUARDO L. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States. |
Título : |
Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Reproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814 |
ISSN : |
1642-431X |
DOI : |
10.1016/j.repbio.2023.100814 |
Idioma : |
Inglés |
Notas : |
Article history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- |
Contenido : |
ABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn MenosABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more ... Presentar Todo |
Palabras claves : |
Fixative solution; Mare; Ovarian tissue quality; Preantral follicle morphometry. |
Asunto categoría : |
L50 Fisiología y bioquímica animal |
Marc : |
LEADER 03765naa a2200301 a 4500 001 1064365 005 2023-11-10 008 2023 bl uuuu u00u1 u #d 022 $a1642-431X 024 7 $a10.1016/j.repbio.2023.100814$2DOI 100 1 $aBORGES, M.A. 245 $aEthanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- 520 $aABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn 653 $aFixative solution 653 $aMare 653 $aOvarian tissue quality 653 $aPreantral follicle morphometry 700 1 $aCURCIO, B.R. 700 1 $aGASTAL, G.D.A. 700 1 $aGHENO, L. 700 1 $aJUNIOR, A.S.V. 700 1 $aCORCINI, C.D. 700 1 $aNOGUEIRA, C.E.W. 700 1 $aAGUIAR, F.L.N. 700 1 $aGASTAL, E.L. 773 $tReproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
05/03/2020 |
Actualizado : |
27/04/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
MONTAÑEZ, A; RIGAMONTI, N.; VICO, S.; SILVA, C.; NUÑEZ, L.; ZERBINO, M.S. |
Afiliación : |
ADRIANA MONTAÑEZ, Universidad de la República Oriental del Uruguay, Facultad de Ciencias, Instituto de Ecología y Ciencias Ambientales, Laboratorio de Microbiología de Suelos, Uruguay.; NATALIA RIGAMONTI, Universidad de la República Oriental del Uruguay, Facultad de Ciencias, Instituto de Ecología y Ciencias Ambientales, Laboratorio de Microbiología de Suelos. Iguá 4225, 11400 Montevideo, Uruguay.; SILVANA VICO, Universidad de la República Oriental del Uruguay, Facultad de Ciencias, Instituto de Ecología y Ciencias Ambientales, Laboratorio de Microbiología de Suelos. Iguá 4225, 11400 Montevideo, Uruguay.; CARLA SILVA, Universidad de la República Oriental del Uruguay, Facultad de Ciencias, Instituto de Ecología y Ciencias Ambientales, Laboratorio de Microbiología de Suelos. Iguá 4225, 11400 Montevideo, Uruguay.; LUCÍA NUÑEZ, Universidad de la República Oriental del Uruguay, Facultad de Ciencias, Instituto de Ecología y Ciencias Ambientales, Laboratorio de Microbiología de Suelos. Iguá 4225, 11400 Montevideo, Uruguay.; MARIA STELLA ZERBINO BARDIER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Influence of aerobic treated manure application on the chemical and microbiological properties of soil. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Spanish Journal of Agricultural Research, 2019, Volume 17, Issue 4, Article number e1104. OPEN ACCESS. Doi: https://doi.org/10.5424/sjar/2019174-14658 |
DOI : |
10.5424/sjar/2019174-14658 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 06 Feb 2019.// Accepted: 16 Dec 2019.The authors would like to thank Andres Peres del Castillo and Christian Decker for their much-appreciated assistance with field work and Sally Bunning for her technical editing of the manuscript. |
Contenido : |
Abstract:
Aim of study: This study evaluated the effect of the application of liquid aerobic treated manure (continuous liquid composting, CLC) on physical, chemical and biological soil properties, with the objective of monitoring changes induced by soil management with CLC as a biofertilizer.
Area of study: Colonia, Uruguay (lat. 34,338164 S, long. 57,222630 W).
Material and methods: Soil?s chemical properties, including nitrogen mineralization potential (NMP) and 15 microbiological properties (microbial biomass carbon, MBC; mesophylic aerobic bacteria; actinobacteria; filamentus fungi; fluorescein diacetate hydrolysis; dehydrogenase; with NMP; acid and alkaline phosphatase; cellulolose degraders; P-solubilizing bacteria; nitrifying; denitrifying and free-living N-fixing microorganisms; glomalin; and soil-pathogenicity index, SPI) were evaluated in two sites with similar cropping history, with one and three years of respective CLC application.
Main results: CLC application had significant effects on soil microbial biomass (p<0.05), soil enzyme (p<0.1) and functional groups activity (p<0.05). SPI decreased in both sites with CLC application. No significant variations were detected for the chemical variables, with the exception of NMP, which was significantly high (p<0.05) in soil treated with CLC at both sites.
Research highlights: The improved biological soil properties analyzed (MBC, soil enzyme activities and SPI, together with NMP) emerged as reasonable indicators to assess and monitor the effects of CLC application. MenosAbstract:
Aim of study: This study evaluated the effect of the application of liquid aerobic treated manure (continuous liquid composting, CLC) on physical, chemical and biological soil properties, with the objective of monitoring changes induced by soil management with CLC as a biofertilizer.
Area of study: Colonia, Uruguay (lat. 34,338164 S, long. 57,222630 W).
Material and methods: Soil?s chemical properties, including nitrogen mineralization potential (NMP) and 15 microbiological properties (microbial biomass carbon, MBC; mesophylic aerobic bacteria; actinobacteria; filamentus fungi; fluorescein diacetate hydrolysis; dehydrogenase; with NMP; acid and alkaline phosphatase; cellulolose degraders; P-solubilizing bacteria; nitrifying; denitrifying and free-living N-fixing microorganisms; glomalin; and soil-pathogenicity index, SPI) were evaluated in two sites with similar cropping history, with one and three years of respective CLC application.
Main results: CLC application had significant effects on soil microbial biomass (p<0.05), soil enzyme (p<0.1) and functional groups activity (p<0.05). SPI decreased in both sites with CLC application. No significant variations were detected for the chemical variables, with the exception of NMP, which was significantly high (p<0.05) in soil treated with CLC at both sites.
Research highlights: The improved biological soil properties analyzed (MBC, soil enzyme activities and SPI, together with NMP) emerged as reasonable indicators to ass... Presentar Todo |
Palabras claves : |
MICROBIOLOGICAL INDICATORS; ORGANIC FERTILIZATION. |
Thesagro : |
ESTIERCOL; FISICA DEL SUELO; Manejo de suelos; NITRIFICACION; QUIMICA DEL SUELO. |
Asunto categoría : |
P30 Ciencia del suelo y manejo del suelo |
Marc : |
LEADER 02744naa a2200289 a 4500 001 1060892 005 2021-04-27 008 2019 bl uuuu u00u1 u #d 024 7 $a10.5424/sjar/2019174-14658$2DOI 100 1 $aMONTAÑEZ, A 245 $aInfluence of aerobic treated manure application on the chemical and microbiological properties of soil.$h[electronic resource] 260 $c2019 500 $aArticle history: Received: 06 Feb 2019.// Accepted: 16 Dec 2019.The authors would like to thank Andres Peres del Castillo and Christian Decker for their much-appreciated assistance with field work and Sally Bunning for her technical editing of the manuscript. 520 $aAbstract: Aim of study: This study evaluated the effect of the application of liquid aerobic treated manure (continuous liquid composting, CLC) on physical, chemical and biological soil properties, with the objective of monitoring changes induced by soil management with CLC as a biofertilizer. Area of study: Colonia, Uruguay (lat. 34,338164 S, long. 57,222630 W). Material and methods: Soil?s chemical properties, including nitrogen mineralization potential (NMP) and 15 microbiological properties (microbial biomass carbon, MBC; mesophylic aerobic bacteria; actinobacteria; filamentus fungi; fluorescein diacetate hydrolysis; dehydrogenase; with NMP; acid and alkaline phosphatase; cellulolose degraders; P-solubilizing bacteria; nitrifying; denitrifying and free-living N-fixing microorganisms; glomalin; and soil-pathogenicity index, SPI) were evaluated in two sites with similar cropping history, with one and three years of respective CLC application. Main results: CLC application had significant effects on soil microbial biomass (p<0.05), soil enzyme (p<0.1) and functional groups activity (p<0.05). SPI decreased in both sites with CLC application. No significant variations were detected for the chemical variables, with the exception of NMP, which was significantly high (p<0.05) in soil treated with CLC at both sites. Research highlights: The improved biological soil properties analyzed (MBC, soil enzyme activities and SPI, together with NMP) emerged as reasonable indicators to assess and monitor the effects of CLC application. 650 $aESTIERCOL 650 $aFISICA DEL SUELO 650 $aManejo de suelos 650 $aNITRIFICACION 650 $aQUIMICA DEL SUELO 653 $aMICROBIOLOGICAL INDICATORS 653 $aORGANIC FERTILIZATION 700 1 $aRIGAMONTI, N. 700 1 $aVICO, S. 700 1 $aSILVA, C. 700 1 $aNUÑEZ, L. 700 1 $aZERBINO, M.S. 773 $tSpanish Journal of Agricultural Research, 2019, Volume 17, Issue 4, Article number e1104. OPEN ACCESS. Doi: https://doi.org/10.5424/sjar/2019174-14658
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